National University

Course:	BIO203A - Introduction to Microbiology Laboratory
Last revised:	Date: September 16, 2009    By: Dr. Huda Makhluf
Textbook:	For the correct edition of the textbook assigned to a specific class, go to: http://www.nutextdirect.com
Course Prerequisite(s): BIO 203
Course Description: This course will introduce the students to laboratory safety and procedures for handling biological specimens. The methods of identification of microorganisms, both microscopic and by diagnostic media, will be emphasized. Students will work with a selection of the most common medically significant microorganisms including bacteria, protists, fungi and parasites. This course emphasizes techniques essential to microbiology including aseptic technique, isolation of a single colony by quadrant streak method, preparation of a pure culture, inoculation and interpretation of select diagnostic tests. In addition, preparation of stained slides and wet mounts, microscopic observations, and the determination of antibiotic susceptibility will be learned. Students will culture and study the normal flora of the throat, skin, and surface of everyday items. An introduction to anaerobic bacteria and culturing techniques will be included. Students will gain an understanding of the difference between sterile conditions and disinfection. This two-month course is a combination of lecture and laboratory activities.
Learning Outcomes: Upon successful completion of this course, the student will be able inoculation and interpretation of select diagnostic tests. In addition, preparation of stained slides and wet mounts, microscopic observations, and the determination of antibiotic susceptibility will be learned. Students will culture and study the normal flora of the throat, skin, and surface of everyday items. An introduction to anaerobic bacteria and to: 1. Learn laboratory safety, conduct, and correct handling of biological specimens. 2. Prepare, observe, and interpret stained slides of bacteria using microscopy. 3. Aseptically transfer, culture bacteria, and isolate individual colonies by using the streak technique. 4. Use differential and selective media to isolate and identify various bacteria. 5. Test microbial susceptibility using different chemical agents. 6. Culture bacteria from common surfaces and substrates. 7. Identify samples of unknown bacteria using deductions and systematic testing.
Course Requirements: CONTENT: 1. General laboratory safety, metric measurement, and correct handling of biological specimens. 2. Learn parts of the compound light microscope, care of, and how to focus. 3. Observe prepared slides of bacteria, fungi, protists, and parasites using all the lenses of a microscope including oil immersion. 4. Learn to use sterile technique regularly in lab activities. 5. Prepare bacterial smears, learn staining techniques to include; simple stain and gram stain to observe different morphologies and staining reactions. 6. Aseptically transfer bacterial cultures to broth, agar slants, spread plate culture, and quadrant streak for colony isolation. 7. From a mixed culture, streak for colony isolation, re-streak to produce a pure culture, and inoculate diagnostic media to identify bacteria. 8. Differentiate between selective and differential media 9. Perform and interpret susceptibility to selected antimicrobial agents. 10.  11. Culture normal flora from the throat and skin using blood agar, learn terminology to describe colony appearance.

Recommended Course Grading (expressed as percentages):

A	96-100	B+	87-89	C+	77-79	D+	67-69
A-	90-95	B	84-86	C	74-76	D	64-66
		B-	80-83	C-	70-73	D-	60-63

Grading Factors:
Each instructor is responsible for identifying several specific grading components and the weight to be assigned to each component. The following are examples only, and are not meant to be all inclusive:

• Weekly quizzes
• Midterm exam(s)
• Homework assignments
• Participation in classroom or chatroom discussions
• Participation in threaded discussions (online only)
• Short papers, term papers, and the like
• Individual and/or group projects (written and possibly oral presentation)
• Case study analysis and discussion
• Field experience report
• Final exam (comprehensive)

Definition of Grades:

Undergraduate Courses
A	Outstanding Achievement	Significantly exceeds standards
B	Commendable Achievement	Exceeds standards
C	Acceptable Achievement	Meets standards
D	Marginal Achievement	Below standards
F	Failing *

Graduate Courses
A	Outstanding Achievement
B	Commendable Achievement
C	Marginal Achievement
D	Unsatisfactory *
F	Failing *

* Students receiving this grade in a course that is required for his/her degree program must repeat the course.

I

Incomplete A grade given at the discretion of the instructor when a student who has completed at least two-thirds of the course class sessions and is unable to complete the requirements of the course because of uncontrollable and unforeseen circumstances. The student must convey these circumstances (preferably in writing) to the instructor prior to the final day of the course. If an instructor decides that an "Incomplete" is warranted, the instructor must convey the conditions for removal of the "Incomplete" to the student in writing. A copy must also be placed on file with the Office of the Registrar until the "Incomplete" is removed or the time limit for removal has passed. An "Incomplete" is not assigned when the only way the student could make up the work would be to attend a major portion of the class when next offered. An "I" that is not removed within the stipulated time becomes an "F." No grade points are assigned. The "F" is calculated in the grade point average.

W

Withdrawal Signifies that a student has withdrawn from a course after beginning the third class session. Students who wish to withdraw must notify their admissions advisor before the beginning of the sixth class session in the case of graduate courses, or before the seventh class session in the case of undergraduate courses. Instructors are not authorized to issue a "W" grade.

Plagiarism:
Plagiarism is the presentation of someone else's ideas or work as one's own. Students must give credit for any information that is not either the result of original research or common knowledge. If a student borrows ideas or information from another author, he/she must acknowledge the author in the body of the text and on the reference page. Students found plagiarizing are subject to the penalties outlined in the Policies and Procedures section of the University Catalog, which may include a failing grade for the work in question or for the entire course. The following is one of many websites that provide helpful information concerning plagiarism for both students and faculty: http://www.indiana.edu/~wts/pamphlets/plagiarism.shtml

Ethics:
Ethical behavior in the classroom is required of every student. The course will identify ethical policies and practices relevant to course topics.

Technology:
Students are expected to be competent in using current technology appropriate for this discipline. Such technology may include word processing, spreadsheet, and presentation software. Use of the internet and e-mail may also be required.

Diversity:
Learning to work with and value diversity is essential in every class. Students are expected to exhibit an appreciation for multinational and gender diversity in the classroom.

Civility:
As a diverse community of learners, students must strive to work together in a setting of civility, tolerance, and respect for each other and for the instructor. Rules of classroom behavior (which apply to online as well as onsite courses) include but are not limited to the following:

• Conflicting opinions among members of a class are to be respected and responded to in a professional manner.
• Side conversations or other distracting behaviors are not to be engaged in during lectures, class discussions or presentations
• There are to be no offensive comments, language, or gestures

Students with Disabilities:
Students seeking special accommodations due to a disability must submit an application with supporting documentation, as explained under this subject heading in the General Catalog. Instructors are required to provide such accommodations if they receive written notification from the University.

Writing Across the Curriculum:
Students are expected to demonstrate writing skills in describing, analyzing and evaluating ideas and experiences. Written reports and research papers must follow specific standards regarding citations of an author's work within the text and references at the end of the paper. Students are encouraged to use the services of the University's Writing Center when preparing materials.

The following website provides information on APA, MLA, and other writing and citation styles that may be required for term papers and the like: http://www.nu.edu/LIBRARY/ReferenceTools/citations.html

National University Library:
National University Library supports academic rigor and student academic success by providing access to scholarly books and journals both electronically and in hard copy. Print materials may be accessed at the Library in San Diego or through document delivery for online and regional students. Librarians are available to provide training, reference assistance, and mentoring at the San Diego Library and virtually for online or regional students. Please take advantage of Library resources:

URL: http://www.nu.edu/library.

Contact the Library:

• RefDesk@nu.edu
• (858) 541-7900 (direct line)
• 1-866-NU ACCESS x7900 (toll free)

Use the Library Training Tools (on the Library Homepage) for additional help

• Recorded class presentations
• Tutorials & Guides (APA/MLA, Peer-Review, and more)